Epimedium extract (monoglycoside 10%.20%)

09/28/2025 16:13:06

Epimedium

Properties: brown-yellow powder
Source: This product selects the dried aerial parts of Herba Epimedii, a wild Berberidaceae plant in the Qinba Mountains
CAS number: 489-32-7Molecular formula: C33H40O15
Molecular weight: 676.66
Melting point: 231℃-232℃
Detection method: HPLC
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Analysis method
1. Thin layer identification method Take 0.5g of Epimedium powder or an appropriate amount of extract, add 10ml of ethanol, soak for 30 minutes, filter, evaporate the filtrate to dryness, and add 1ml of ethanol to the residue to dissolve it, which is the test solution. Weigh the icariin reference substance, dissolve it with methanol and dilute it to 0.1mg/ml, which is the reference solution. 10 μl of the test solution and the reference solution were taken and spotted on the same silica gel H thin layer plate with sodium carboxymethyl cellulose as the adhesive. Ethyl acetate-butanone-formic acid-water (10:1:1:1) was used as the developing agent. After development, the plate was taken out, dried, and examined under a UV lamp (365 nm). In the chromatogram of the test sample, the same dark red spots appeared at the corresponding positions of the chromatogram of the reference substance;
2. Spray with aluminum chloride test solution, and then examine under a UV lamp (365 nm), and the same orange-red fluorescent spots appeared. Determination of the content of total flavonoids in epimedium and its extracts by spectrophotometry Accurately weigh the reference substance icariin and prepare a 10ug/ml standard solution with methanol. Accurately weigh an appropriate amount of the powder of this product (equivalent to about 1 mg of icariin), ultrasonicate with 25 ml of methanol for 30 minutes, dilute to a 50 ml volumetric flask, and filter with a 0.45 μm microporous filter membrane to obtain the test solution. Take the test solution and the reference solution respectively, use the reagent as the blank, measure the absorbance at a wavelength of 270 nm, and calculate the result.

3. HPLC determination of icariin content in epimedium and its extracts Chromatographic conditions and system suitability test: ODS25uC18 (4mm×150mm) chromatographic column; mobile phase: acetonitrile-water (30:70); detection wavelength: 270 nm; flow rate: 0.8 ml/min. The theoretical plate number is not less than 1500 based on icariin. Preparation of reference solution: Accurately weigh the reference icariin and prepare a 0.1 mg/ml solution with methanol. Preparation of test solution: Accurately weigh an appropriate amount of the powder of this product (equivalent to about 1 mg of icariin), ultrasonicate with 25 ml of methanol for 30 minutes, make up to the volume in a 50 ml volumetric flask, and filter with a 0.45 μm microporous filter membrane to obtain the test solution. Determination method: Inject 10 μl of the sample test solution and the reference solution into the HPLC instrument respectively, and calculate by the area external standard method.
Pharmacological action
1. Effect on sexual function Epimedium extract has a certain effect on promoting gonadal function. Flavonoid components such as icariin have the effect of tonifying the kidney and strengthening yang. 30-1000 μg/l can promote the secretion of estradiol by follicular granulosa cells, and 1000 μg/l can promote the secretion of corticosterone by adrenal cortical cells. At the same time, EPS can increase the level of gonadal hormones, mainly the level of FSH.
2. Improve the body's immune function Epimedium 50% methanol extract can inhibit lymphocyte transformation. Total flavonoids of Epimedium can make "Yang deficiency" mice form cell function and antibodies, and significantly enhance the immune function of human T cells. Both EPS and EI have immunomodulatory effects.
3. Antioxidant EPS and EI have antioxidant activity, which can enhance the activity of antioxidant enzymes and reduce the effect of free radical products.
4. Anti-aging effect Epimedium extract can resist aging and prolong life by affecting cell passage, prolonging growth period, regulating immune and secretory systems, improving body metabolism and organ functions.
5. Cardiovascular effect The non-amino acid part of Chemicalbook in the alcohol extract of Epimedium can significantly increase the coronary flow of isolated rabbit hearts. Decoction and alcohol extract have a hypotensive effect. Icariin can directly relax vascular smooth muscle and dilate coronary arteries, femoral arteries and cerebral arteries. The mechanism of action is to inhibit the influx of extracellular calcium ions in vascular smooth muscle cells.
6. Anti-inflammatory and anti-allergic effects Epimedium methanol extract can significantly reduce the swelling of egg white "arthritis" in rats and reduce the increased capillary permeability of rabbits caused by histamine. It can also inhibit allergic asthma in guinea pigs caused by histamine and acetylcholine.

7. Effect on bone growth Epimedium extract has the ability to inhibit the activity of osteoclasts, while promoting the function of osteoblasts, increasing the formation of calcified bones, and promoting the synthesis of DNA in bone marrow cells. It can not only prevent osteoporosis caused by castrated rats, but also prevent osteopenia and osteoporosis caused by hormones. Other studies have shown that epimedium injection has a significant promoting effect on the growth of chicken embryo femurs in vitro.
8. Other effects Epimedium crude extract has expectorant, antitussive and antiasthmatic effects.